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Detail page - EMDB-1333Sixfold averaged tail of bacteriophage K1E
in 'single particle' state, at 16.4 A resolution
| Database / ID | EM DATA BANK (EMDB) / 1333 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Sample Name / Map Annotation | Sixfold averaged tail of bacteriophage K1E Sixfold averaged tail of bacteriophage K1E | ||||||||
| Authors | Leiman PG, Battisti AJ, Bowman VD, Stummeyer K, Muhlenhoff M, Gerardy-Schahn R, Scholl D, Molineux IJ | ||||||||
| Movies | |||||||||
| EMDB figure |  | ||||||||
| Related Entries |
Cite: data citing same article | ||||||||
| Last Update | 2007-03-06 | ||||||||
| Map Release Date | 2007-07-18 | ||||||||
| EMDB Sites |  EMDB @EBI (EU) EMDB @RCSB (USA) |
| Article | J. Mol. Biol., Vol. 371, Issue 3, Page 836-49, Year 2007 |
|---|---|
| Title | The structures of bacteriophages K1E and K1-5 explain processive degradation of polysaccharide capsules and evolution of new host specificities. |
| Authors | Petr G Leiman, Anthony J Battisti, Valorie D Bowman, Katharina Stummeyer, Martina Mühlenhoff, Rita Gerardy-Schahn, Dean Scholl, Ian J Molineux |
| Affiliation | Department of Biological Sciences, Purdue University, 915 W. State Street, West Lafayette, IN 47907-2054, USA. |
| Chemicals | Bacterial Capsules, DNA, Viral, Viral Proteins |
| Keywords |  Bacterial Capsules, Bacteriophages, Capsid, Cryoelectron Microscopy, DNA Packaging, DNA, Viral, Escherichia coli, Evolution, Genome, Viral, Models, Molecular, Sequence Homology, Amino Acid, Species Specificity, Static Electricity, Viral Proteins, Virion |
| Links | PubMed: 17585937 DOI: 10.1016/j.jmb.2007.05.083 |
| File |  emd_1333.map ( map file in CCP4 format, 21808 KB ) | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Projections |     | ||||||||||||||||||||
| Density | 
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| Data Type | float (32-bit) | ||||||||||||||||||||
| Space Group Number | 1 | ||||||||||||||||||||
| Map Geometry |
| ||||||||||||||||||||
| Unit Cell | A = 524.480 A , B = 524.480 A , C = 524.480 A , alpha = 90.000 degrees , beta = 90.000 degrees , gamma = 90.000 degrees | ||||||||||||||||||||
| Pixel Spacing | X = 2.98 A , Y = 2.98 A , Z = 2.98 A | ||||||||||||||||||||
| CCP4 map header info | under construction! | ||||||||||||||||||||
| Details | EMAN 3/20/2006 12:41 tail-unbin_4.spCreatedby MAPMAN V. 050302/7.8.1 at Thu Apr 2012:41:51 2006 for A. Nonymous Created byIMAGIC:CCP4 image = tail-unbin_4.map 06-03-2022:49:17 | ||||||||||||||||||||
| Annotation Details | Sixfold averaged tail of bacteriophage K1E |
| Name | Sixfold averaged tail of bacteriophage K1E |
|---|---|
| Aggregation State | single particle |
| Number of Components | 1 |
| Experimental Mass | 8 MDa |
| Theoretical Mass | 8 MDa |
| Mass-estimation Method | Primary sequence |
| Component #1: virus - phage K1E tail | |
| Scientific name | bacteriophage K1E tail |
| Common Name | phage K1E tail |
| Theoretical Mass | 8 MDa |
| Experimental Mass | 8 MDa |
| Details | sixfold averaged |
| Host Category | BACTERIA(EUBACTERIA) |
| Host Species | Escherichia coli |
| Class | VIRION |
| Isolate | SPECIES |
| Enveloped | No |
| Empty | No |
| Sample Preparation | |
| Buffer | Details: 50 mM Tris HCl pH 7.5, 100 mM NaCl, 8 mM MgSO4 Sample Conc: 1 mg/ml pH: 7.5 |
| Staining | none |
| Sample Support Details | quantifoil grid |
| Vitrification | |
| Cryogen Name | ETHANE |
| Humidity | 80 |
| Temperature | 300 Kelvin |
| Instrument | Vitrobot |
| Imaging | |
| Microscope | FEI/PHILIPS CM300FEG/T |
| Date | 2005-05-10 |
| Electron Gun | |
| Electron Source | FIELD EMISSION GUN |
| Accelerating Voltage | 300 kV |
| Electron Dose | 25 e/A**2 |
| Illumination Mode | SPOT SCAN |
| Lens | |
| Magnification | Nominal: 45000 X, Calibrated: 47000 X |
| Nominal Cs | 2.0 mm |
| Imaging Mode | BRIGHT FIELD |
| Defocus | 700 nM - 3300 nM |
| Specimen Holder | |
| Holder | Side entry liquid nitrogen-cooled cryo specimen holder ( GATAN LIQUID NITROGEN ) |
| Temperature | 100 Kelvin |
| Camera | |
| Detector | Kodak SO163 film |
| Image Scans | |
| Number of Digital Images | 122 |
| Sampling Size | 7 microns |
| Quant Bit Number | 12 |
| Scanner | ZEISS SCAI |
| 3D Reconstruction | |
| Algorithm | weighted back-projection |
| Software | Spider |
| CTF Correction | Each particle |
| Resolution By Author | 16.4 |
| Resolution Method | FSC at 0.4 cut-off |
| EM Data Set ( single particle ) | |
| Number of Projections | 6105 |
| Data from EMDB | |||
| Header Information | emd-1333.xml ( XML format, 5,970 byte ) | ||
| Map Data | emd_1333.map.gz ( 13,715,191 byte )Details | ||
| FTP site (EMDB mirror) | EMD-1333 | ||
| Data from PDBj EM Navigator | |||
| movie #1 |
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| movie #2 |
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